Microbial　polyhydroxyalkanoate　(PHA)　production　serves　as　a　substitute　for　petroleum-based　plastics.　Enriching　mixed　microbial　cultures　(MMCs)　with　the　capacity　to　store　PHA　is　a　key　precursor　for　low-cost　PHA　production.　This　study　investigated　the　impact　of　carbon　types　on　enrichment　outcomes.　Three　MMCs　were　separately　fed　by　acetate　sodium,　glucose,　and　starch　as　an　enriching　carbon　source,　and　were　exposed　to　long-term　aerobic　dynamic　feeding　(ADF)　periods.　The　PHA　production　capacity,　kinetics　and　stoichiometry　of　the　enrichments,　the　PHA　composition,　and　the　microbial　diversity　and　community　composition　were　explored　to　determine　carbon　and　enrichment　correlations.　After　350-cycle　enriching　periods　under　feast-famine　(F-F)　regimes,　the　MMCs　enriched　by　acetate　sodium　and　glucose　contained　a　maximum　PHA　content　of　64.7%　and　60.5%　cell　dry　weight　(CDW).　The　starch-enriched　MMC　only　had　27.3%　CDW　of　PHA.　High-throughput　sequencing　revealed　that　non-PHA　bacteria　survived　alongside　PHA　storing　bacteria,　even　under　severe　F-F　selective　pressure.　Genus　of　Pseudomonas　and　Stappia　were　the　possible　PHA　accumulating　bacteria　in　acetate-enriched　MMC.　Genus　of　Oceanicella,　Piscicoccus　and　Vibrio　were　found　as　PHA　accumulating　bacteria　in　glucoseenriched　MMC.　Vibrio　genus　was　the　only　PHA　accumulating　bacteria　in　starch-enriched　MMC.　The　community　diversity　and　composition　were　regulated　by　the　substrate　types.