To　explore　the　altered　different　expression　of　miRNAs　and　the　mechanisms　underlying　the　relapse　and　metastasis　of　pancreatic　cancer.　The　most　differentially　expressed　miRNAs　were　analyzed　by　gene　ontology　(GO)　term　analysis,　Kyoto　encyclopedia　of　genes　and　genomes　(KEGG)　pathway　analysis　and　protein　interaction　analysis.　The　potentially　regulated　target　genes　of　the　most　differentially　expressed　miRNAs　were　also　analyzed　further　by　GO　term　analysis　and　KEGG　pathway　analysis,　and　quantitated　by　qRT-PCR.　In　total,　we　found　12　miRNAs　displayed　at　least　a　30-fold　increase　or　decrease　in　expression　of　carcinoma　and　relapse　vs.　para-carcinoma　human　pancreatic　cancer　(C/R　vs.　P).　In　addition,　our　study　found　that　pancreatic　cancer　was　related　to　pathways　in　cancer,　including　Jak-STAT　signaling　pathway,　MAPK　signaling　pathway　and　PPAR　signaling　pathway.　The　differential　expressed　miRNAs　and　their　predicted　target　genes　that　involved　in　Jak-STAT　signaling　pathway,　MAPK　signaling　pathway　and　PPAR　signaling　pathway　indicating　their　potential　roles　in　pancreatic　carcinogenesis　and　progress.