In　this　study,　the　physicochemical　characteristics　of　the　dissolved　organic　matter　(DOM)　in　simulated　raw　water,　corresponding　filter　backwash　water　(FBWW)　and　treated　water　at　three　recycling　ratios　of　2,　5,　and　8%,　including　control　without　FBWW　were　evaluated　using　bulk　properties,　in　addition　to　advanced　characterization　by　molecular　weight　distribution　(MWD),　hydrophobicity,　and　fluorescence.　The　DOM　characteristics　of　raw　water　and　FBWW　were　primarily　identified　and　compared,　and　it　was　found　that　DOM　in　FBWW　was　roughly　hydrophilic　as　compared　with　raw　water,　and　the　FBWW　was　more　refractory　due　to　a　substantial　increment　of　low-MW　(＜　3　KDa).　Additionally,　humic-like　fluorophore　(Peak　A)　and　protein-like　fluorophore　(Peak　T1),　with　maxima　at　the　Ex/Em　wavelengths　of　245.0-265.0　nm/400.0-435.0　nm,　280.0-305.0　nm/305.0-345.0　nm,　respectively,　were　the　two　main　components　in　raw　water　and　FBWW.　Recycling　trial　results　indicated　that　DOM　removal　efficiencies　were　improved　with　comparison　to　control.　Specifically,　all　chemical　fractions　except　hydrophobic　neutral　could　enhance　removal,　but　MWD　was　highly　depended　on　the　physical　nature　of　raw　water　and　FBWW.　Fluorescence　spectra　of　treated　water　indicated　recycling　FBWW　could　effectively　improve　humic-like　substance　(Peak　A)　removal,　but　the　protein-like　matter　(Peak　T1)　was　resistant　to　elimination　or　was　enriched.