To　achieve　the　stable　partial　nitrification　of　municipal　wastewater,　activated　sludge　with　high　ammonia-oxidizing　bacteria　(AOB)　content　and　low　nitrite-oxidizing　bacteria　(NOB)　content　were　immobilized　in　a　polyvinyl　alcohol　filler.　The　effects　of　different　levels　of　dissolved　oxygen　(DO)　on　the　activity　of　AOB　and　NOB　in　the　filler　with　temperature　changes　at　the　initial　ammonia　concentration　of　approximately　100　mg　L(-1)were　investigated.　At　25　degrees　C,　when　the　DO　concentration　was　greater　than　5　mg　L-1,　the　O-2-limiting　condition　inside　the　filler　was　destroyed　as　the　demand　for　oxygen　in　AOB　was　certain,　and　resulted　in　enhanced　NOB　activity.　At　15　degrees　C,　the　DO　concentration　was　not　a　key　factor　in　determining　the　NOB　activity　due　to　the　negative　effect　of　temperature　on　NOB　activity.　The　immobilized　filler　reactor　of　municipal　wastewater　achieved　a　nitrite　accumulation　rate　(NAR)　of　＞86.7　and　＞82%　at　24-26　degrees　C　and　14-16　degrees　C,　respectively.　Low　temperatures　did　not　deteriorate　the　stable　partial　nitrification　performance.　The　total　nitrogen　(TN)　removal　efficiency　of　the　immobilized　filler　reactor　was　21.7-26.1%　and　10.3-15.3%　at　24-26　degrees　C　and　14-16　degrees　C,　respectively.　The　TN　removal　efficiency　and　NAR　in　municipal　wastewater　were　higher　as　compared　to　simulated　wastewater,　indicating　that　the　organic　carbon　in　municipal　wastewater　enhanced　nitrate　reduction　by　denitrification.　High-throughput　sequencing　analysis　showed　that　denitrifying　bacteria　and　nitrifying　bacteria　were　identified　as　the　predominant　bacteria　genera,　while　the　dominant　species　of　NOB　wasNitrobacter.　This　study　is　a　viable　approach　to　promoting　partial　nitrification　in　municipal　WWTPs.