The　analysis　of　single　living　cells,　including　intracellular　delivery　and　extraction,　is　essential　for　monitoring　their　dynamic　biochemical　processes　and　exploring　intracellular　heterogeneity.　However,　owing　to　the　2D　view　in　bright-field　microscopy　and　optical　distortions　caused　by　the　cell　shape　and　the　variation　in　the　refractive　index　both　inside　and　around　the　cells,　achieving　spatially　undistorted　imaging　for　high-precision　manipulation　within　a　cell　is　challenging.　Here,　an　accurate　and　visual　system　is　developed　for　single-cell　spatial　manipulation　by　correcting　the　aberration　for　simultaneous　bright-field　triple-view　imaging.　Stereo　information　from　the　triple　view　enables　higher　spatial　resolution　that　facilitates　the　precise　manipulation　of　single　cells.　In　the　bright　field,　we　resolved　the　spatial　locations　of　subcellular　structures　of　a　single　cell　suspended　in　a　medium　and　measured　the　random　spatial　rotation　angle　of　the　cell　with　a　precision　of　+/-　5　degrees.　Furthermore,　we　demonstrated　the　visual　manipulation　of　a　probe　to　an　arbitrary　spatial　point　of　a　cell　with　an　accuracy　of　＜1　pixel.　This　novel　system　is　more　accurate　and　less　destructive　for　subcellular　content　extraction　and　drug　delivery.