This　paper　aimed　to　study　the　six　chemical　components　of　Polygoni　Multiflori　Radix　(gallic　acid,　quercetin,　luteolin,　kaempferol,　resveratrol,　apigenin).　By　the　established　pregnane　X　receptor　(human　pregnant　X　receptor,　PXR)　CYP3A4　mediated　drug　induced　rapid　screening　technique,　the　effect　of　chemical　components　on　the　cell　activity　was　detected　by　MTS　cell　method,　and　the　value　of　IC₅₀　was　calculated.　The　dual　luciferase　reporter　system　was　used　to　co-transfect　PXR　reporter　gene　expression　vector　containing　transcriptional　regulation　and　CYP3A4　with　HepG2　cells,　with　10　μmol·L⁻¹　rifampicin　(RIF)　as　a　positive　control,　and　10　μmol·L⁻¹　of　ketoconazole　(TKZ)　as　negative　control.　Gallic　acid,　quercetin,　luteolin,　kaempferol,　apigenin,　resveratrol(5,　10,　20　μmol·L⁻¹)　were　used　to　incubate　for　24　h,　and　the　luciferase　activity　was　detected.　The　results　showed　that　when　plasmid　pcDNA3.1　was　co-transfected　with　pGL4.17-CYP3A4,　gallic　acid　and　resveratrol　had　an　inhibitory　effect　on　the　regulation　of　CYP3A4,　and　quercetin,　luteolin,　kaempferol　had　an　inductive　effect　on　CYP3A4;　when　pcDNA3.14-PXR　was　co-transfected　with　pGL4.17-CYP3A4,　quercetin,　luteolin,　kaempferol,　apigenin,　resveratrol　had　an　inductive　effect.　To　sum　up,　the　6　reported　liver　injury　components　had　inhibitory　or　activating　effects　on　CYP3A4.　After　PXR　plasmid　was　involved,　5　components　had　an　inductive　effect　on　CYP3A4,　and　the　inductive　effects　of　2　components　were　significantly　different.　In　this　experiment,　we　found　that　2　kinds　of　potential　liver　injury　components　in　Polygoni　Multiflori　Radix　had　been　induced　by　CYP3A4,　which　was　achieved　through　PXR　regulation.　It　suggested　that　attention　shall　be　paid　to　potential　drug　interactions　when　combined　with　Polygoni　Multiflori　Radix,　so　as　to　improve　the　safety　and　efficacy.