Wastewater　containing　2,4,6-trichlorophenol　(2,4,6-TCP)　is　highly　toxic　and　causes　harmful　effects　on　aquatic　ecosystems　and　human　health.　In　this　study,　wastewater　containing　high　levels　of　2,4,6-TCP　was　successfully　co-metabolized　by　introducing　municipal　domestic　wastewater　(MDW)　as　the　co-catabolic　carbon　source.　The　concentration　of　degraded　2,4,6-TCP　increased　from　0　to　208.71　mg/L　by　adjusting　the　influent　MDW　volume　during　a　150-day-long　operation.　An　MDW　dose　of　500　mL　was　found　optimal,　with　an　average　concentration　of　250　mgCOD/L.　Unlike　the　long-term　experiment,　changing　the　MDW　adding　mode　in　a　typical　cycle　further　increased　the　concentration　of　2,4,6-TCP　removed　to　317　mg/L.　The　main　MDW　components,　such　as　the　sugars,　VFAs,　and　slowly　biodegradable　organic　substances,　improved　2,4,6-TCP　degradation,　achieving　a　TOC　removal　efficiency　of　90.98%　and　a　dechlorination　efficiency　of　100%.　The　MDW　level　did　not　change　the　2,4,6-TCP　degradation　rate　(mu(TCP))　in　a　typical　cycle　compared　to　the　single　carbon　source,　and　the　mu(TCP)　remained　at　a　high　level　of　50　mg　2,4,6-TCP/h.　Macrogenetic　analysis　demonstrated　that　MDW　addition　promoted　the　growth　of　43　bacterial　genera　(41.49%)　responsible　for　2,4,6-TCP　degradation　and　intermediates＇　metabolism.　The　key　genes　for　2,4,6-TCP　metabolism　(pcpA,　chqB,　mat-r,　pcaf,　pcaF,　and　fadA)　were　detected　in　the　activated　sludge,　which　were　distributed　among　the　43　genera.　To　conclude,　this　study　proposes　a　new　carbon　source　for　co-metabolism　to　treat　2,4,6-TCP-polluted　wastewater.