The　mixture　of　partial　AGS　and　flocculent　sludge　in　continuous　flow　reactors　were　operated　with　periodic　famine　(PF)　strategy　and　continuous　feast　(CF)　strategy　to　reveal　the　impact　of　the　feast-famine　strategies　on　cultivation　of　AGS　and　the　dynamics　of　microbial　communities.　The　experimental　results　showed　that　the　mature　AGS　were　cultivated　with　PF　and　CF　strategy　on　the　31st　and　the　71st　days　respectively,　which　was　the　result　of　good　extra　cellular　polymeric　substance　(EPS)　secretion　with　PF　strategy.　It　could　accelerate　the　formation　of　microbial　aggregates　due　to　the　conditions　of　periodic　famine.　High-level　denitrification　with　PF　strategy　via　utilization　of　SMP　was　examined　by　EEM-PARAFAC　on　cycle　test.　The　high-throughput　pyrosequencing　showed　that　the　dominant　bacteria　with　PF　strategy　involved　functional　bacteria　of　nutrient　removal　and　EPS　secreting　bacteria,　while　the　dominant　bacteria　were　fast-growing　heterotrophic　organisms　with　CF　strategy　.　(c)　2021　Elsevier　B.V.　All　rights　reserved.