As　a　new　field,　there　are　still　many　unknowns　and　challenges　in　the　study　of　nanozymes,　such　as　the　mechanism　of　the　reaction　catalyzed　by　nanozymes　and　the　precise　control　of　the　catalytic　activity　of　nanozymes.　We　found　that　Pd@Pt　nanoparticles　(NPs)　have　trienzyme　mimetic　activity,　including　peroxidase-like,　catalase-like,　and　oxidase-like　activity.　More　importantly,　we　observed　that　the　catalytic　performance　can　be　modulated　by　DNA.　After　systematic　studies,　the　potential　mechanisms　of　DNA　modulation　of　nanozymes　were　through　local　coordination,　van　der　Waals　forces,　and　hydrophobic　forces,　constructing　an　interface　modulation　in　the　Pd@Pt　NP　enzyme-like　system.　Based　on　the　surface-interface　effect　between　DNA　and　Pd@Pt　NP　nanozymes,　a　colorimetric　sensor　was　constructed　for　rapid,　accurate　and　selective　analysis　of　Salmonella　by　using　a　specific　aptamer.　After　optimization,　the　limit　of　detection　was　calculated　to　be　~58　CFU　mL(-1).　These　results　indicated　that　the　catalytic　activity　of　Pd@Pt　NP　nanozymes　can　be　modulated　by　DNA,　and　the　surface-interface　effect　has　potential　in　the　application　of　analytical　assays.