While　single-cell　mass　spectrometry　can　reveal　cellular　heterogeneity　and　the　molecular　mechanisms　of　intracellular　biochemical　reactions,　its　application　is　limited　by　the　insufficient　detection　sensitivity　resulting　from　matrix　interference　and　sample　dilution.　Herein,　we　propose　an　intact　living-cell　electrolaunching　ionization　mass　spectrometry　(ILCEI-MS)　method.　A　capillary　emitter　with　a　narrow-bore,　constant-inner-diameter　ensures　that　the　entire　living　cell　enters　the　MS　ion-transfer　tube.　Inlet　ionization　improves　sample　utilization,　and　no　solvent　is　required,　preventing　sample　dilution　and　matrix　interference.　Based　on　these　features,　the　detection　sensitivity　is　greatly　improved,　and　the　average　signal-to-noise　(S/N)　ratio　is　about　20　:　1　of　single-cell　peaks　in　the　TIC　of　ILCEI-MS.　A　high　detection　throughput　of　51　cells　per　min　was　achieved　by　ILCEI-MS　for　the　single-cell　metabolic　profiling　of　multiple　cell　lines,　and　368　cellular　metabolites　were　identified.　Further,　more　than　4000　primary　single　cells　digested　from　the　fresh　multi-organ　tissues　of　mice　were　detected　by　ILCEI-MS,　demonstrating　its　applicability　and　reliability.