Enhanced　biological　phosphorus　removal　(EBPR)　is　an　economical　and　sustainable　process　for　phosphorus　removal　from　wastewater.　Despite　the　widespread　application　of　EBPR　for　low-strength　domestic　wastewater　treatment,　limited　investigations　have　been　conducted　to　apply　EBPR　to　the　high-strength　wastewaters,　particularly,　the　integration　of　EBPR　and　the　short-cut　nitrogen　removal　process　in　the　one-stage　system　remains　challenging.　Herein,　we　reported　a　novel　proof-of-concept　demonstration　of　integrating　EBPR　and　nitritation　(oxidation　of　ammonium　to　nitrite)　in　a　one-stage　sequencing　batch　reactor　to　achieve　simultaneous　high-strength　phosphorus　and　short-cut　nitrogen　removal.　Excellent　EBPR　performance　of　effluent　0.8　&　PLUSMN;　1.0　mg　P/L　and　＞99%　removal　efficiency　was　achieved　fed　with　synthetic　high-strength　phosphorus　wastewater.　Long-term　sludge　acclimation　proved　that　the　dominant　polyphosphate　accumulating　organisms　(PAOs),　Candidatus　Accumulibacter,　could　evolve　to　a　specific　subtype　that　can　tolerate　the　nitrite　inhibition　as　revealed　by　operational　taxonomic　unit　(OTU)-based　oligotyping　analysis.　The　EBPR　kinetic　and　stoichiometric　evaluations　combined　with　the　amplicon　sequencing　proved　that　the　Candidatus　Competibacter,　as　the　dominant　glycogen　accumulating　organisms　(GAOs),　could　well　coexist　with　PAOs　(15.3-24.9%　and　14.2-33.1%,　respectively)　and　did　not　deteriorate　the　EBPR　performance.　The　nitrification　activity　assessment,　amplicon　sequencing,　and　functional-based　gene　marker　quantification　verified　that　the　unexpected　nitrite　accumulation　(10.7-21.0　mg　N/L)　in　the　high-strength　EBPR　system　was　likely　caused　by　the　nitritation　process,　in　which　the　nitrite-oxidizing　bacteria　(NOB)　were　successfully　out-selected　(＜0.1%　relative　abundance).　We　hypothesized　that　the　introduction　of　the　anaerobic　phase　with　high　VFA　concentrations　could　be　the　potential　selection　force　for　achieving　nitritation　based　on　the　literature　review　and　our　preliminary　batch　tests.　This　study　sheds　light　on　developing　a　new　feasible　technical　route　for　integrating　EBPR　with　short-cut　nitrogen　removal　for　efficient　high-strength　wastewater　treatment.