Realizing　the　quick　enrichment　and　development　of　denitrifying　phosphorus　accumulating　organisms　(DPAOs)　in　actual　household　wastewater　and　industrial　nitrate　wastewater　has　significant　research　significance.　In　this　study,　a　novel　operation　mode　of　anaerobic-oxic-anoxic　(AOA)　was　adopted　to　successfully　realize　the　enrichment　and　cultivation　of　DPAOs　in　urban　domestic　wastewater.　Adjusting　influent　COD　to　PO43--P　ratio,　shortening　the　aerobic　time　and　decreasing　the　aeration　volume　were　conducive　to　select　DPAOs　in　microbial　populations.　The　system　was　operated　for　180　days　and　the　DPAOs　were　well　enriched　during　the　stable　operation　with　the　percentage　of　Dechloromonas　increased　to　5.1　%.　Accordingly,　the　effluent　PO43--P　was　＜　0.3　mg　P/L,　the　removal　efficiency　of　phosphorus　was　96.9　%　and　the　removal　efficiency　of　nitrate　was　92.5　%.　Above　all,　DPR　can　be　successfully　applied　to　AOA　systems　with　good　phosphorus　removal　performance.