The　label-free　detection　and　analysis　of　cancer　cells　using　portable　biosensing　devices　is　crucial　and　promising.　In　this　study,　a　novel　reusable　biosensing　platform　with　a　microfluidic-like　based　on　terahertz　plasmonic　metasurfaces　utilizing　graphene　integrated　with　an　all-silicon　groove　for　detecting　liquid　live　cancer　cells　was　developed.　The　proposed　biosensor　platform　stands　out　because　it　can　differentiate　between　the　concentrations　of　three　types　of　cancer　cells　by　monitoring　changes　in　resonance　intensity　and　phase　difference.　The　minimum　concentration　for　identification　was　reduced　to　as　low　as　5　×　104　cells/mL.　We　effectively　constructed　two-dimensional　optical　intensity　cards　using　continuous　wavelet　transforms,　which　presented　a　more　accurate　approach　for　the　recognition　and　determination　of　the　three　types　of　cancer　cells.　Our　proposed　biosensors　show　great　potential　for　the　determination　and　recognition　of　label-free　cancer　cells　in　aqueous　environments　as　alternatives　to　non-immune　biosensing　technology.　©　2024