The　pH　and　the　conductivity　of　the　anode　solution　and　the　cathode　solution　were　tested,　and　the　open　circuit　voltage　of　the　MFC　was　analyzed　in　this　paper,　providing　a　theoretical　basis　to　improve　the　performance　of　MFC.　The　start-up　time　of　the　MFC　is　shorter　when　bacteria　are　domesticated　and　cultured　The　open　circuit　voltage　is　more　stable　and　higher　under　the　conditions　of　the　constant　temperature,　high　temperature　and　high　concentration　of　the　substrate.　The　open　circuit　voltage　is　reduced　when　the　product　accumulates　in　the　anode　solution.　The　pH　difference　of　the　anode　solution　and　the　cathode　solution　is　positive　related　to　the　current　in　the　circuit.　The　conductivity　of　the　anode　solution　reduces,　and　the　conductivity　of　the　cathode　solution　increases,　and　ion　in　the　anode　solution　migrate　to　the　cathode　solution　when　the　MFC　runs　normally.　The　speed　of　the　ion　migration　is　positive　related　to　the　current　in　the　circuit.