Under　room　temperature,　for　learning　the　effects　of　aeration　and　temperature　on　start-up　of　shortcut　nitrification,　secondary　effluent　which　is　got　from　domestic　wastewater　after　A/O　process　is　treated　in　a　sequencing　batch　biofilm　reactor(SBBR).　In　the　initial　experiment,　the　SBBR　is　filmed　naturally,　and　then　nitrite　oxidizing　bacteria(NOB)are　restrained　by　keeping　high　concentration　of　FA　and　low　concentration　of　DO　in　order　to　make　ammonia　oxidizing　bacteria(AOB)be　the　dominant　bacterium.　The　tested　results　prove　that　the　nitration　biofilm　in　SBBR　is　cultivated　and　enriched　successfully　in　12　days,　it　is　fast　to　get　film.　On　the　assumption　that　shortcut　nitrification　is　not　impacted,　NOB　could　be　restrained　effectively　in　low　concentration　of　DO,　it　is　favorable　for　AOB　to　be　the　superior　bacterium　in　the　reactor　and　ammonia　nitrogen　transformation　of　influent　is　not　impacted.　On　the　condition　of　low　concentration　of　ammonia　nitrogen,　lower　temperature　restrains　the　activity　of　AOB,　and　nitrite　accumulation　rate　could　not　increase　steadily　by　shortening　the　aeration　time.