Nanomaterials　combined　with　antibiotics　exhibit　synergistic　effects　and　have　gained　increasing　interest　as　promising　antimicrobial　agents.　In　this　study,　vancomycin-modified　magnetic-based　silver　microflowers　(Van/Fe3O4@　SiO2@Ag　microflowers)　were　rationally　designed　and　prepared　to　achieve　strong　bactericidal　ability,　a　wide　antimicrobial　spectrum,　and　good　recyclability.　High-performance　Fe3O4@SiO2@Ag　microflowers　served　as　a　multifunction-supporting　matrix　and　exhibited　sufficient　magnetic　response　property　due　to　their　200　nm　Fe3O4　core.　The　microflowers　also　possessed　a　highly　branched　flower-like　Ag　shell　that　provided　a　large　surface　area　for　effective　Ag　ion　release　and　bacterial　contact.　The　modified-vancomycin　layer　was　effectively　bound　to　the　cell　wall　of　bacteria　to　increase　the　permeability　of　the　cell　membrane　and　facilitate　the　entry　of　the　Ag　ions　into　the　bacterium,　resulting　in　cell　death.　As　such,　the　fabricated　Van/Fe3O4@SiO2@Ag　microflowers　were　predicted　to　be　an　effective　and　environment-friendly　antibacterial　agent.　This　hypothesis　was　verified　through　sterilization　of　Gram-negative　Escherichia　coli　and　Gram-positive　methicillin-resistant　Staphylococcus　aureus,　with　minimum　inhibitory　concentrations　of　10　and　20　mu　g　mL(-1),　respectively.　The　microflowers　also　showed　enhanced　effect　compared　with　bare　Fe3O4@SiO2@Ag　microflowers　and　free-form　vancomycin,　confirming　the　synergistic　effects　of　the　combination　of　the　two　components.　Moreover,　the　antimicrobial　effect　was　maintained　at　more　than　90%　after　five　cycling　assays,　indicating　the　high　stability　of　the　product.　These　findings　reveal　that　Van/Fe3O4@SiO2@Ag　microflowers　exhibit　promising　applications　in　the　antibacterial　fields.