Background:　The　extremely　low-frequency　electromagnetic　field　(ELF-EMF),　mainly　emitted　by　electric　transmission　lines　and　household　electronic　appliances,　is　becoming　a　worldwide　health　risk.　It　is　imperative　to　investigate　the　biological　impacts　of　ELF-EMF　and　to　identify　products　that　are　resistant　to　the　radiation　from　50　Hz　ELF-EMF.　In　this　study,　we　investigated　the　biological　impacts　of　apoptosis　caused　by　50　Hz　Power　line　ELF-EMF　and　the　protective　effects　of　Vit　C　and　Vit　E.　Methods:　We　conducted　this　study　in　Beijing,　China　in　2013.　B95-8　and　Balb/c-3T3　cells　were　divided　into　a　sham　group,　an　expo　group　and　3　expo　groups　in　which　the　cells　were　preincubated　with　various　concentrations　of　Vit　C　and　Vit　E.　Then,　all　of　the　cells　were　exposed　to　50　Hz　Power　line　ELF-EMF　and　examined　for　apoptosis.　The　cells　were　collected　for　apoptosis　detection　after　exposure.　Results:　The　percent　of　cells　that　undergoing　apoptosis　and　preincubated　with　various　concentrations　of　Vit　C　and　Vit　E　were　significantly　lower　than　in　the　Expo　group.　Conclusion:　Vit　C　and　Vit　E　exert　significant　protective　effects　from　50　Hz　ELF-EMF　radiation.　The　optimal　protective　concentrations　of　Vit　C　and　Vit　E　are　10　mu　mol/L　and　25　mu　mol/L,　respectively.　The　protective　effect　of　vitamins　was　more　apparent　for　Balb/c-3T3　cells　than　B95-8　cells.