Background:　Simultaneous　measurement　of　four-limb　blood　pressures　can　improve　the　accuracy　of　cardiovascular　disease　diagnosis.　This　study　aims　to　investigate　the　association　of　simultaneously　measured　four-limb　blood　pressures　with　cardiovascular　function　as　the　non-invasive　diagnostic　method　of　cardiovascular　disease　in　primary　care.　Methods:　229　subjects　(62　males,　mean　age,　63.50　+/-　11.13　years;　167　females,　mean　age,　59.47　+/-　7.33　years)　were　enrolled.　Four-limb　blood　pressure　measurements　were　simultaneously　performed　using　a　blood　pressure　and　pulse　monitor　device　in　the　supine　position.　Cardiac　functional　parameters　were　also　measured　by　using　a　cardiac　hemodynamic　detector　in　the　same　position.　Data　were　statistically　analyzed　with　SPSS15.0.　Results:　The　mean　age　of　the　229　subjects　was　60.56　+/-　8.68　years.　Cardiovascular　functional　parameters　decreased　with　age　and　body　mass　index　(BMI),　only　the　total　peripheral　resistance　(TPR)　was　in　contrast.　Age,　BMI,　left　ankle　diastolic　pressure　(LADP),　high　arm　mean　arterial　pressure　(HARMAP),　left　arm　diastolic　pressure　(LARDP)　and　right　ankle　diastolic　pressure　(RADP)　were　significantly　correlated　with　cardiovascular　functional　parameters.　Cardiovascular　functional　parameters　have　significant　differences　with　inter-arm　difference　in　systolic　blood　pressure　(SBP)　between　＞=　10　and　＜　10　mmHg,　inter-ankle　difference　in　SBP　between　＞=　15　and　＞=　20　mmHg,　inter-ankle　difference　in　SBP　between　＞=　15　and　＜　10　mmHg　and　right　ankle　brachial　index　(RABI)　between　＜=　0.9　and　＞=　1.0.　After　excluding　99　hypertensive　patients,　a　part　of　cardiovascular　functional　parameters　has　still　significant　differences　with　inter-arm　difference　in　SBP　between　＜=　10　and　＞=　15　mmHg　and　RABI　between　＜=　0.9　and　＞=　1.0.　Conclusion:　Age,　BMI,　LADP,　HARMAP,　LARDP　and　RADP　were　the　determinants　of　cardiovascular　functional　parameters.　In　addition,　a　part　of　cardiovascular　functional　parameter　is　associated　with　inter-arm　difference　in　SBP　＞=　10　mmHg,　inter-ankle　difference　in　SBP　＞=　15　mmHg　and　RABI　＜=　0.9,　while　these　differences　still　existed　after　excluding　99　hypertensive　patients.　Hence,　simultaneous　measurement　of　four-limb　blood　pressures　has　become　feasible　and　useful　approach　to　the　non-invasive　diagnostic　method　of　cardiovascular　disease　in　primary　care.