Metal-organic　frameworks　(MOFs)　are　synthesized　via　the　self-assembling　combination　of　organic　ligands　and　inorganic　metals　or　metal-oxo　units　(secondary　building　units,　SBUs)　using　strong　bonds　to　form　a　porous　open　crystalline.　Because　of　their　outstanding　thermal　stability,　chemical　stability　and　designability,　MOFs　are　successfully　applied　in　various　fields,　such　as　gas　absorption,　separation,　purification,　catalysis,　as　well　as　templates　for　various　functional　materials.　MOFs　are　also　used　as　column　materials　for　gas　or　liquid　chromatography　separation.　In　this　paper,　the　studies　about　MOFs　used　as　stationary　phases　in　gas　chromatography　and　high-performance　liquid　chromatography　were　reviewed　according　to　the　separated　various　analytes.　We　summarized　the　separation　effects　of　different　MOFs　for　different　substances,　especially　focusing　on　the　separation　mechanism　of　MOFs.　The　key　influence　factors　on　the　chromatographic　separation,　such　as　pore　diameter,　functional　groups　and　unsaturated　metal　sites　of　MOFs,　were　expounded.　Then,　the　challenges　of　MOFs　as　stationary　phases　in　gas　and　high-performance　liquid　chromatography　were　discussed,　which　include　the　mechanism,　chiral　separation,　the　limited　MOFs　in　chromatographic　separation.　The　future　research　in　this　field　should　focus　on　defining　separation　mechanism,　improving　their　stability　and　synthesizing　the　MOFs　with　special　function.