CRF01_AE　is　one　of　the　four　dominant　HIV-1　strains　circulating　in　China.　In　this　study,　we　performed　genetic　and　phenotypic　analyses　using　a　total　of　60　full-length　envelope　gene　clones　from　14　HIV-1-infected　individuals　in　the　Beijing　area.　Among　the　60　sequences　analyzed,　32　have　a　complete　open　reading　frame　(ORF),　whereas　the　others　contain　premature　stop　codons.　The　phylogenetic　tree　analysis　suggested　that　all　of　the　sequences　maintained　a　close　relationship　with　the　CRF01_AE　strain.　Most　of　the　potential　N-linked　glycosylation　sites　(PNGS)　were　located　within　the　V1/V2,　V4,　C2,　or　C3　regions.　In　relation　to　gp41,　the　majority　of　the　glycosylation　sites　were　located　in　the　ectodomain.　The　32　env　genes　that　contained　intact　ORFs　were　used　to　construct　Env-pseudotyped　viruses,　and　eight　strains　that　resulted　in　high　titers　were　further　studied.　All　the　eight　strains　used　CCR5　as　the　co-receptor　for　infection,　and　they　were　sensitive　to　neutralization　by　the　broadly　neutralizing　monoclonal　antibodies,　including　VRC_01,　PG9,　PG16,　and　NIH45-46,　but　they　were　insensitive　to　2G12.　Notably,　seven　of　these　eight　strains　lacked　a　glycan　at　residues　295　or　332　(or　both),　suggesting　that　these　two　PNGSs　play　an　important　role　in　2G12　binding　and　neutralization.　In　addition,　the　pseudoviruses　were　more　sensitive　to　neutralization　by　plasma　isolated　from　individuals　infected　with　subtypes　CRF01_AE　and　CRF07/08_BC,　suggesting　the　occurrence　of　a　cross-neutralizing　antibody　profile　between　these　two　strains.　These　findings　are　likely　to　have　important　implications　for　the　design　of　an　effective　HIV　vaccine　and　relevant　therapeutic　drugs.