The　hierarchical-structured　superhydrophilic　poly(ethylenimine)/poly(acrylic　acid)　(PEI/PAA)calcium　silicate　hydrate　(CSH)　Multilayered　membranes　(PEI/PAA-CSH)(n)　were　prepared　as　aqueous　nanofiltration　(NF)　membrane,　and　then　they　were　transformed　into　super　hydrophobic　organic　solvent　nanofiltration　(OSN)　membranes　by　one-step　modification　of　trimethylperfluorinatedsilane　(PFTS).　Investigation　on　surface　structures　and　properties　of　these　multilayered　membranes　(PEI/PAA-CSH)(n)　indicated　that　the　hierarchical-structured　(PEI/PAA-CSH)(n)　multilayered　membrane　produced　by　in　situ　incorporation　of　CSH　aggregates　into　PEI/PAA　multilayers　facilitated　its　one-step　transformation　from　superhydrophilicity　into　superhydrophobicity.　Both　of　the　superwetting　membranes　showed　better　nanofiltration　performances　for　retention　of　dyes　of　water　and　ethanol　solution,　respectively.　Moreover,　the　long-term　performance　and　antifouling　behaviors,　investigated　by　retention　of　methyl　blue　(MB),　bovine　serum　albumin　(BSA),　and　humic　acid　(HA)　aqueous　water　solution　and　nonaqueous　ethanol　solution　indicated　that　both　of　the　superhydrophilic　and　superhydrophobic　membrane　showed　higher　stability　and　excellent　antifouling　property.