MicroRNAs　are　highly　conserved　non-coding　RNAs　that　regulate　gene　expression　at　the　post-transcriptional　level,　and　play　pivotal　roles　in　cancer　development　and　progression.　miR-100　has　been　reported　to　be　significantly　downregulated　in　a　variety　of　cancers,　including　esophageal　cancer.　However,　the　role　of　miR-100　in　human　esophageal　cancer　has　not　been　fully　elucidated.　We　demonstrated　that　overexpression　of　miR-100　in　esophageal　cancer　cells　markedly　inhibited　cell　proliferation,　migration　and　invasion　as　well　as　tumor　growth.　We　subsequently　showed　that　CXCR7　is　a　direct　target　gene　of　miR-100.　Our　results　indicated　that　miR-100　plays　a　tumor　suppressor　role　in　esophageal　cancer　and　suggest　its　potential　application　for　esophageal　cancer　treatment.