A　series　of　dead-end　unstirred　microfiltration　experiments　were　conducted.　Impact　of　different　operating　conditions　(trans-membrane　pressure　(TMP),　temperature,　and　concentration)　on　the　specific　cake　resistance　(SCR)　using　yeast　suspension　and　polyethersulfone　membranes　of　0.1m　was　systematically　studied.　The　results　showed　that　TMP,　temperature,　and　concentration　have　a　significant　influence　on　the　SCR.　The　SCR　increased　with　the　increasing　concentration　and　TMP,　while　decreased　with　the　increasing　temperature.　The　sequence　of　average　accumulated　impact　of　the　operating　conditions　on　the　SCR　was　TMP　(54.6%)＞temperature　(-24.2%)＞concentration　(21.1%).　The　total　accumulated　impact　of　operating　conditions　on　the　SCR　was　44.5%.　This　result　provides　the　basis　for　process　optimization　and　process　modeling.