To　achieve　nitrifying　bacteria　(ammonia-oxidizing　bacteria　(AOB)　and　nitrite-oxidizing　bacteria　(NOB))　growth,　activated　sludge　from　the　A(2)/O　process　in　a　wastewater　treatment　plant　(WWTP)　was　used　as　seed　sludge　to　guide　the　industrial　culture　of　nitrifying　bacteria　for　ammonia-contaminated　wastewater　treatment.　The　bacterial　community　was　enriched　in　a　fully　automatic　miniature　fermenter　by　an　alternating　mode　of　intermittent　operation　mode　in　the　daytime　and　a　continuous　ammonia　feeding　mode　for　intermittent　operation　during　the　night.　The　results　indicated　that　the　nitrifying　bacteria　community　can　be　enriched　by　this　alternating　operation　mode,　resulting　in　a　maximum　ammonia　oxidation　rate　reaching　approximately　110　mg　L-1　h(-1)　and　a　nitrite　oxidation　rate　of　approximately　50　mg　L-1　h(-1).　High-throughput　sequencing　demonstrated　that　Nitrosomonas　and　Nitrospira　belonging　to　AOB　and　NOB,　respectively,　were　predominant　in　the　activated　sludge　after　25　days　at　a　ratio　extending　from　2.94%　to　60.66%.