Anaerobic　ammonium　oxidation　(anammox)　immobilized　particles　were　added　to　a　reactor　as　biocatalyst　to　start　an　up-flow　anammox　reactor.　The　kinetic　characteristics　of　the　immobilized　particles　were　studied　using　batch　tests.　The　results　demonstrated　that　using　immobilized　particles　as　the　biocatalyst　succeeded　in　starting　the　anammox　reactor　in　49　d.　The　removal　rates　of　NH4+-N　and　NO2--N　were　80.7　and　83.1%,　respectively.　The　total　nitrogen　removal　load　was　0.505　kg　m(-3)　d(-1).　The　results　of　the　kinetic　characteristics　study　demonstrated　that　the　half-saturation　constants　of　the　immobilized　particles　for　ammonium　and　nitrite　were　1.57　and　1.505　mmol　L-1,　respectively.　These　constants　were　lower　than　those　of　flocculent　anammox　sludge　(FAS),　which　indicated　that　the　immobilized　particles　utilize　the　substrate　better　in　adverse　environments.　The　half-suppression　constants　of　the　immobilized　particles　for　ammonia　and　nitrite　were　724.2　and　66.65　mmol　L-1,　respectively,　and　higher　than　those　of　FAS.　Thus,　the　immobilized　particles　could　survive　longer　in　high-concentration　substrates.　As　a　biocatalyst　in　a　reactor,　the　anammox　immobilized　particles　exhibited　robust　kinetic　characteristics　and　effectiveness,　which　are　important　to　the　development　of　new　biocatalyst　techniques　for　anammox.