Enterovirus　71　(EV71)　and　coxsackievirus　A16　(CVA16)　are　members　of　the　Picornaviridae　family　and　are　considered　the　main　causative　agents　of　hand,　foot　and　mouth　disease　(HFMD).　In　recent　decades　large　HFMD　outbreaks　caused　by　EV71　and　CVA16　have　become　significant　public　health　concerns　in　the　Asia-Pacific　region.　Vaccines　and　antiviral　drugs　are　unavailable　to　prevent　EV71　and　CVA16　infection.　In　the　current　study,　a　chimeric　antibody　targeting　a　highly　conserved　peptide　in　the　EV71　VP4　protein　was　isolated　by　using　a　phage　display　technique.　The　antibody　showed　cross　neutralizing　capability　against　EV71　and　CVA16　in　vitro.　The　results　suggest　that　this　phage　display　derived　antibody　will　have　great　potential　as　a　broad　neutralizing　antibody　against　EV71　and　CVA16　after　affinity　maturation　and　humanization.