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摘要:
In this paper, we report the improvement of PCR microfluidic chip efficiency achieved by coating the inner surface of steel capillary microchannel with a 22-A mu m film of the ultraviolet-solidified NOA61 using a device invented by us. Our results indicate that with this treatment, the roughness of the inside wall of steel capillary was improved from Ra = 0.921 to Ra = 0.254. The contact angle was decreased from about 95A degrees to 56A degrees, and the surface hydrophobicity was also increased. The flow pressure for performing the real-time PCR in the microfluidic chip with modified surface was reduced by twofold (2.11/1) and that resulted in a substantially increased efficiency of PCR. A modification of the microchannel interior surface improved the quality of the on-chip integrated PCR procedure.
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来源 :
CELL BIOCHEMISTRY AND BIOPHYSICS
ISSN: 1085-9195
年份: 2015
期: 2
卷: 72
页码: 605-610
2 . 6 0 0
JCR@2022
ESI学科: BIOLOGY & BIOCHEMISTRY;
ESI高被引阀值:254
JCR分区:3
中科院分区:4