A　mechanism　model　of　enzyme　injection　biosensor　was　established　and　the　accuracy　of　the　model　was　verified　through　experiments　in　this　study.　The　parameters　of　the　reaction　model　were　determined　by　fitting　the　voltage　data　with　model　and　the　voltage　data　was　obtained　by　detecting　1　and　2　mg　mL(-1)　glucose　solution.　Accuracy　of　the　model　was　verified　by　comparing　the　fitting　curve　and　the　actual　curve.　The　fitting　curve　was　obtained　by　taking　the　value　of　3　mg　mL(-1)　into　the　model　and　the　actual　data　was　detected　by　actual　sensors.　Experimental　results　showed　that　the　liquid　enzyme　Michaelis　constant　K-m　was　1.97,　the　correlation　coefficient　between　mathematical　model　and　the　actual　sensor　working　model　was　0.998.