High-risk　human　papillomaviruses　(HPV)　have　been　associated　with　many　human　cancers　in　clinical　studies.　Integration　of　HPV　into　the　human　genome　is　a　suspected　etiological　factor　in　the　induction　of　some　HPV-associated　cancers.　The　characteristics　of　HPV　integration　in　certain　HPV-integrated　cancer　cells　remain　unclear.　In　this　study,　ten　HPV-associated　carcinoma　cell　lines　were　evaluated　for　the　presence,　genotype,　and　integration　status　of　HPV　by　nested　polymerase　chain　reaction.　The　HPV　genome　did　not　insert　in　the　genome　of　a　mammary　cancer　cell　line　(MCF7),　adrenal　neuroblastoma　cell　line　(NH-6),　or　three　esophageal　carcinoma　cell　lines　(KYSE150,　KYSE450　and　KYSE140).　HPV　type　18　DNA　did　infect　cell　lines　of　tongue　cancer　(Tca83),　hepatocellular　carcinoma　(Hep　G2),　and　lung　carcinoma　(A549),　but　the　HPV　type　18　genes　were　not　transcribed　into　mRNA.　However,　HPV　type　18　integrated　into　the　genomes　of　the　esophageal　carcinoma　cell　lines　EC9706　and　EC109,　and　the　integration　sites　for　both　cell　lines　were　in　loci　8q24,　which　is　a　gene　desert　area　adjacent　to　fragile　sites.　We　speculate　that　HPV　transcripts　are　more　likely　to　integrate　near　highly　susceptible　fragile　sites.　This　study　suggests　that　HPV　integration　is　still　a　significant　issue　that　needs　to　be　fully　examined　and　can　possibly　be　used　as　individualized　biomarkers　for　the　early　diagnosis　of　HPV-related　cancers.