Current　models　of　stem　cell　biology　assume　that　glioma　stem　cells　reside　at　the　apices　of　hierarchies　and　differentiate　into　non-stem　progeny　in　a　unidirectional　manner.　However,　here　we　found　an　opposite　phenomenon　that　glioma　stem　spheres　could　induce　adjacent　non-stem　glioma　cells　to　spontaneously　dedifferentiate　into　stem-like　cells　in　low　temperature　condition.　In　low　temperature　condition,　it　has　been　reported　that　mild　hypothermia　could　induce　pluripotent　stem　cells　in　hESC　and　iPSC.　However,　till　now,　its　effects　on　glioma　stem　cells　were　still　unknown.　In　this　study,　tracking　the　non-stem　cells,　we　found　that　they　could　be　attracted　by　stem　spheres,　and　finally　enter　the　stem　spheres　to　become　a　member　of　stem　spheres　in　vitro.　However,　these　induced　stem-like　cells　positive　of　CD133　and　Nestin　markers　could　not　form　an　obvious　sphere.　To　better　understand　the　genetic　differences　of　the　stem　spheres　and　stem-like　cells　underlying　the　change　of　microenvironment,　we　carried　out　Cytokine　antibody　array,　Cancer　PathwayFinder　PCR　array,　and　miRNA　chip　array,　which　demonstrated　that　lots　of　cytokines,　mRNAs,　and　miRNAs　involved　in　this　microenvironmental　change.　In　this　study,　the　most　important　discovery　by　us　was　that　we　found　GSCs　sphere　cores,　which　has　been　found　to　have　strong　proliferative　capacity,　and　be　able　to　100　%　form　a　big　GSCs　sphere.　We　hope　these　findings　can　change　our　past　concepts,　and　be　help　to　the　further　research　on　gliomas　stem　cells,　and　GSCs　sphere　cores　can　be　defined　as　the　primitive　stem　cells　for　further　research.