Ag-nanoparticle　films　were　deposited　on　glass　and　silicon　substrates　by　evaporation　of　a　colloidal　solution.　Periodic　structures　were　created　in　these　Ag　nanoparticle　deposits　by　ultraviolet　nanosecond　laser　interference　lithography　(LIL).　We　find　that　the　periodic　structure　and　size　of　Ag　nanoparticles　in　these　oriented　arrays　can　be　controlled　by　varying　laser　pulse　energy　as　well　as　the　number　of　pulses　used　in　irradiation.　The　effect　of　these　parameters　on　the　overall　morphology　of　these　deposits　is　shown　to　be　promising　as　a　new　technique　for　the　engineering　of　sub-micron　nanoparticle　structures.　Ag　films　with　laser-induced　sub-micron　periodic　structures　were　evaluated　as　substrates　for　surface-enhanced　Raman　spectroscopy　using　rhodamine　B　adsorbed　from　a　10(-8)　M　solution.　Enhancement　factors　were　found　to　be　＞10(6)　those　found　in　conventional　Raman　scattering　and　10　times　greater　than　those　from　standard　Ag-nanoparticle　films.　These　results　indicate　that　the　LIL　method　has　promise　in　the　nanostructuring　of　Ag-nanoparticle　films　for　ultrasensitive　SERS　biosensing　substrates.