In　this　study,　a　sequencing　batch　reactor　(SBR)　was　used　to　achieve　advanced　nitrogen　removal　by　simultaneous　Anammox　and　denitrification　processes.　During　the　entire　experiment,　the　Anammox　microorganisms　aggregated　in　the　reactor　as　wall　growth.　Nitrogen　removal　was　improved　due　to　the　reduction　of　nitrate,　and　the　maximum　total　nitrogen　(TN,　including　ammonia,　nitrite　and　nitrate　nitrogen)　removal　efficiency　of　97.47%　was　obtained　at　C/N　of　2.　However,　the　sequentially　increased　organic　matter　resulted　in　a　poor　TN　removal　performance　due　to　the　suppression　of　Anammox.　Fortunately,　the　Anammox　activity　completely　resumed　quickly　after　stopping　dosing　organic　matter.　PCR　analysis　results　revealed　that　the　Anammox　bacteria　gene　copy　number　was　not　significantly　reduced　during　the　inhibition,　which　might　explain　the　quick　recover.　(C)　2014　Elsevier　Ltd.　All　rights　reserved.