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作者:

Chen, Yushu (Chen, Yushu.) | Bai, Bo (Bai, Bo.) | Zhang, Shujiang (Zhang, Shujiang.) | Ye, Jing (Ye, Jing.) | Chen, Yi (Chen, Yi.) | Zeng, Yanjun (Zeng, Yanjun.)

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摘要:

The present study was conducted in order to explore the mechanisms whereby parathyroid hormone (PTH) maintains in vitro proliferation of bone marrow mesenchymal stem cells (BMSCs). Bone marrow was isolated from Sprague Dawley (SD) rat femurs, cultured in vitro, and passaged using a cell adherent culture method. The BMSC proliferation was evaluated by the methyl thiazolyl tetrazolium (MTT) assay and the fluorescence intensity of calcium ions in BMSCs was analyzed by laser scanning confocal microscopy (LSCM). Our results show that BMSC proliferation in the experimental group treated with PTH was more significant than controls. The calcium ion fluorescence intensity in BMSCs was significantly higher for the experimental group as compared to the control group. For each group, there was significant difference in the fluorescence intensity of calcium ions in BMSCs between 7 d and 14 d. In conclusion, parathyroid hormone increased the fluorescence intensity of calcium ions in BMSCs, which might represent a key mechanism whereby BMSC proliferation is maintained.

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作者机构:

  • [ 1 ] [Chen, Yushu]Guangzhou Med Univ, Affiliated Hosp 1, Orthoped Implantat Key Lab Guangdong Prov, Dept Orthopaed Surg, Guangzhou 510120, Guangdong, Peoples R China
  • [ 2 ] [Bai, Bo]Guangzhou Med Univ, Affiliated Hosp 1, Orthoped Implantat Key Lab Guangdong Prov, Dept Orthopaed Surg, Guangzhou 510120, Guangdong, Peoples R China
  • [ 3 ] [Zhang, Shujiang]Guangzhou Med Univ, Affiliated Hosp 1, Orthoped Implantat Key Lab Guangdong Prov, Dept Orthopaed Surg, Guangzhou 510120, Guangdong, Peoples R China
  • [ 4 ] [Chen, Yi]Guangzhou Med Univ, Affiliated Hosp 1, Orthoped Implantat Key Lab Guangdong Prov, Dept Orthopaed Surg, Guangzhou 510120, Guangdong, Peoples R China
  • [ 5 ] [Ye, Jing]1st People Hosp Yunfu, Dept Orthopaed Surg, Yunfu 527300, Peoples R China
  • [ 6 ] [Zeng, Yanjun]Beijing Univ Technol, Biomech & Med Informat Inst, Beijing 100022, Peoples R China

通讯作者信息:

  • [Bai, Bo]Guangzhou Med Univ, Affiliated Hosp 1, Orthoped Implantat Key Lab Guangdong Prov, Dept Orthopaed Surg, 151 Yanjiang Rd, Guangzhou 510120, Guangdong, Peoples R China

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来源 :

BIOMED RESEARCH INTERNATIONAL

ISSN: 2314-6133

年份: 2014

卷: 2014

ESI学科: BIOLOGY & BIOCHEMISTRY;

ESI高被引阀值:285

JCR分区:3

中科院分区:3

被引次数:

WoS核心集被引频次: 4

SCOPUS被引频次: 7

ESI高被引论文在榜: 0 展开所有

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