The　objective　of　this　study　was　to　investigate　the　diagnostic　significance　of　EBV　antibody　combined　detection　for　nasopharyngeal　carcinoma　(NPC)　in　a　high　incidence　region　of　southern　China.　Two　hundred　and　eleven　untreated　NPC　patients,　203　non-NPC　ENT　patients,　and　210　healthy　controls　were　recruited　for　the　study.　The　titers　of　VCA/IgA　and　EA/IgA　were　assessed　by　immunoenzyme　assay,　and　the　levels　of　Rta/IgG　and　EBNA1/IgA　were　determined　by　enzyme-linked　immunosorbent　assay.　The　levels　of　VCA/IgA,　EA/IgA,　Rta/IgG　and　EBNA1/IgA　demonstrated　no　association　with　gender　or　age　(p＞0.05).　The　receiver　operating　characteristic　curve　and　the　area　under　the　curve　were　used　to　evaluate　the　diagnostic　value.　The　sensitivity　of　VCA/IgA　(98.1%)　and　the　specificity　of　EA/IgA　(98.5%)　were　the　highest.　When　a　logistic　regression　model　was　used　to　combine　the　results　from　multiple　antibodies　to　increase　the　accuracy,　the　combination　of　VCA/IgA+Rta/IgG,　whose　area　under　the　curve　was　0.99,　had　the　highest　diagnostic　efficiency,　and　its　sensitivity,　specificity　and　Youden　index　were　94.8%,　98.0%　and　0.93　respectively.　The　data　suggest　that　the　combination　of　VCA/IgA+Rta/IgG　may　be　most　suitable　for　NPC　serodiagnosis.