The　inactivation　of　a　gram-negative　sulfonamide　antibiotic　resistant　bacteria　(ARB)　HLS.6　and　removal　of　intracellular　antibiotic　resistance　gene　(ARG,　sul1)　and　class　I　integrase　gene　(intI1)　by　nanoscale　zero-valent　iron　(nZVI)　and　sulfide-modified　nZVI　(S-nZVI)　with　different　S/Fe　molar　ratios　were　investigated　in　this　study.　The　S-nZVI　with　high　sulfur　content　(S/Fe　=　0.05,　0.1,　0.2)　was　superior　to　nZVI　and　the　treatment　effect　was　best　when　S/Fe　was　0.1.　The　ARB　(2　x　10(7)　CFU/mL)　could　be　completely　inactivated　by　1.12　g/L　of　S-nZVI　(S/Fe　=　0.1)　within　15　min,　and　the　removal　rates　of　intracellular　sull　and　intI1　reached　up　to　4.39　log　and　4.67　log　at　60　min,　respectively.　Quenching　experiments　and　flow　cytometry　proved　that　reactive　oxygen　species　and　adsorption　were　involved　in　the　ARB　inactivation　and　target　genes　removal.　Bacterial　death　and　live　staining　experiments　and　transmission　electron　microscopy　showed　that　the　ARB　cell　structure　and　intracellular　DNA　were　severely　damaged　after　S-nZVI　treatment.　This　study　provided　a　potential　alternative　method　for　controlling　the　antibiotic　resistance　in　aquatic　environment.