Human　umbilical　cord-derived　mesenchymal　stem　cells　(hUC-MSCs)　hold　great　potential　for　their　therapeutic　use　in　various　clinical　diseases.　Many　publications　have　reported　on　human　blood-derived　alternatives　to　animal　serum　for　culturing　mesenchymal　stem　cells,　such　as　human　serum,　allogenic　umbilical　cord　blood　serum,　and　human　platelet　derivatives.　However,　it　is　not　clear　whether　human　umbilical　cord　blood　plasma　(UCBP),　as　the　surplusage　of　umbilical　cord　blood　mesenchymal　stem　cell　extraction,　could　be　used.　In　this　study,　in　order　to　make　the　best　of　umbilical　cord　blood,　the　human　UCBP　was　dialyzed　to　replace　fetal　bovine　serum　(FBS)　in　the　culture　medium.　hUC-MSCs　were　cultured　in　the　new　medium.　Cell　growth　rate,　specific　biomarkers,　and　differentiation　properties　were　detected　to　characterize　the　cell　proliferation　and　MSC-specific　properties.　The　hUC-MSCs　cultured　in　such　derived　medium　were　verified　with　proliferation　rate,　cluster　differentiation　markers,　cell　cycle,　as　well　as　differentiation　capabilities.　Such　dialyzed　human　UCBP　is　fully　comparable　with,　if　not　superior　to,　FBS　in　deriving　and　culturing　hUC-MSCs.