DNA　damage　induced　from　perfluorooctane　sulfonate　(PFOS)　was　further　developed　on　a　nano-porous　bionic　interface.　The　interface　was　formed　by　assembling　DNA　on　nano-gold　particles　which　were　embedded　in　a　nano-porous　overoxidized　polypyrrole　film　(OPPy).　Atomic　force　microscopy,　scanning　electron　microscope　and　electrochemical　investigations　indicate　that　OPPy　can　be　treated　to　form　nano-pore　structures.　DNA　damage　due　to　PFOS　was　proved　using　electrochemistry　and　X-ray　photoelectron　spectroscopy　(XPS)　and　was　investigated　by　detecting　differential　pulse　voltammetry　(DPV)　response　of　methylene　blue　(MB)　which　was　used　as　electro-active　indicator　in　the　system.　The　current　of　MB　attenuates　obviously　after　incubation　of　DNA　in　PFOS.　Moreover,　electrochemical　impedance　spectroscopy　(EIS)　demonstrates　that　PFOS　weakens　DNA　charge　transport.　The　tentative　binding　ratio　of　PFOS:　DNA　base　pair　was　obtained　by　analyzing　XPS　data　of　this　system.　(C)　2013　Elsevier　B.　V.　All　rights　reserved.