Aim:　We　aimed　to　evaluate　the　effectiveness　of　the　application　of　activated　autologous　monocytes/macrophages　(Mo/Mp)　on　wound　healing　in　diabetic　rats.　Methods:　Sixty　male　SD　rats　were　equally　divided　into　the　following:　control　group　(normal,　nondiabetic),　PBS-treated　diabetic　group,　and　tumor　necrotic　factor　alpha　(TNF-alpha)　plus　interferon-g　(IFN-gamma)-stimulated　or　unstimulated　Mo/Mp-treated　diabetic　group.　Full-thickness　round　wounds　(1　cm　x　1　cm)　were　created　in　the　right　hind　foot　of　rats　and　the　wounds　were　treated　with　PBS　or　Mo/Mp　on　day　1　after　injury.　In　the　following　14　days,　the　percentage　of　wound　contraction　was　measured,　histologic　examination　was　performed　with　hematoxylin　and　eosin　staining,　and　vascular　endothelial　growth　factor　(VEGF)　in　the　wound　was　evaluated　by　Western　blot　analysis.　Results:　Diabetic　rats　exhibited　impaired　wound　healing　with　delayed　angiogenesis　and　VEGF　expression.　The　early　application　of　TNF-alpha　plus　IFN-g-stimulated　autologous　Mo/Mp　to　diabetic　wounds　significantly　improved　the　delayed　wound　healing　through　the　stimulation　of　angiogenesis　and　re-epithelization,　as　well　as　restoring　the　defect　in　VEGF　expression.　Conclusions:　Mo/Mp　activated　by　TNF-alpha　and　IFN-gamma　promotes　diabetic　wound　healing　and　normalizes　the　defect　in　VEGF　regulation　associated　with　diabetes-induced　skin-repair　disorders.　(C)　2013　Elsevier　Ireland　Ltd.　All　rights　reserved.