Objective:　In　order　to　study　the　ability　of　nano-hydroxyapatite　artificial　bone　in　repair　of　large　segmental　bone　defect　and　to　evaluate　the　value　of　radionuclide　bone　imaging　in　monitoring　the　bone＇s　repairing　in　the　bone　defect.　Methods:　The　animal　model　of　bone　defect　was　made　on　the　bilateral　radius　of　24　(New　repaired　with　HA　artificial　bone)　with　12　rabbits　in　each　group.　The　ability　of　bone　defect　repair　was　evaluated　by　using　radionuclide　bone　imaging　at　2,　4,　8　and　12　weeks　postoperatively.　Results:　The　counts　of　regions　of　interest　(ROI)　measure　and　the　target　to　non-target　ratios　(T/NT)　of　the　two　groups　indicate　that　the　experimental　group　stimulated　more　bone　formation　than　that　of　the　control　group.　The　differences　of　the　bone　reconstruction　ability　were　statistically　significant　(P　＞　0.05).　Conclusions:　The　nano-HA　artificial　has　good　bone　conduction,　it　can　be　used　for　the　treatment　of　bone　defects.　Radionuclide　imaging　may　be　an　effective　and　first　choice　method　for　the　early　monitoring　of　the　bone＇s　reconstruction.