Background:　We　aimed　to　guide　clinical　nursing　by　studying　the　relationship　between　intestinal　acute　graft-versus-host　disease　and　intestinal　infection　after　hematopoietic　stem　cell　transplantation.　Material/Methods:　We　present　an　effective　nursing　method　by　comparing　and　analyzing　the　degree,　duration　time,　and　volume　of　diarrhea,　and　the　distribution　of　pathogens　in　44　patients　who　developed　intestinal　aGVHD　after　hematopoietic　stem　cell　transplantation　(24　patients　with　no　intestinal　infection).　Results:　21.4%　of　patients　with　grade　I-II　intestinal　aGVHD　developed　into　intestinal　infection　and　87.5%　of　patients　with　grade　III-IV　intestinal　aGVHD　developed　into　intestinal　infection　(P＜0.05).　Higher　mortality　was　found　in　the　grade　III-IV　intestinal　aGVHD　patients　with　intestinal　infection.　Patient　age　had　no　effect　on　the　incidence　of　GVHD　according　to　our　data　(P＜0.05).　We　found　remarkable　differences　in　the　amount　and　duration　of　diarrhea　between　patients　with　and　without　intestinal　infection　(P＜0.05).　The　most　common　pathogens　cultivated　were　Candida　glabrata　(24%)　and　Candida　albicans　(22.67%).　Conclusions:　The　incidence　of　intestinal　infection　increased　remarkably　after　intestinal　aGVHD　occurred.　Severe　aGVHD　can　easily　lead　to　fungus　infection.　Nursing　care　can　decrease　the　incidence　of　intestinal　infection　in　aGVHD.