In　texturing　dentin　surface　by　KrF　laser　with　the　fluences　of　200-800　mJ/cm(2),　it　is　observed　the　irradiated　surface　topography　has　different　characteristics　with　that　irradiated　under　high　fluence　above　1000　mJ/cm(2)　reported　in　previous　studies.　The　formation　threshold　fluence　of　the　microstructure　consisted　of　erected　microcylinders　was　determined　at　400　mJ/cm(2).　By　scanning　electron　microscopy　including　fine　examination　of　a　single　microstructure　unit　under　high　magnification,　the　microstructure　formation　and　evolution　tending　to　the　fashion　of　high　fluence　were　given.　Analysis　of　the　ablation　details　captured　by　high-speed　photography　confirmed　the　development　mechanism　of　the　texturing　microstructure.　Raman　analysis　showed　the　composition　of　the　laser　textured　dentin　underwent　changes.　Although　the　texturing　is　related　to　the　characteristics　of　the　dentin　tissue,　laser　parameters　play　a　decisive　role　in　the　controlling　of　the　shape　and　the　height　of　the　three-dimensional　microstructure.　The　combination　of　our　work　and　the　previous　work　by　Sivakumar　et　al.　[J.　Laser　Appl.　18(4),　330-333　(2006)　and　Lasers　Med.　Sci.　21,　160-164　(2006)]　demonstrates　laser　fabrication　of　the　three-dimensional　microstructure　with　different　topography　features　on　dentin　surface　is　controllable.　(C)　2013　Laser　Institute　of　America.