Anatase　TiO2　nanosheets　(ATNs)　are　successfully　prepared　by　a　biomimetic　layer-by-layer　titania　mineralization　approach,　and　the　electrochemical　performance　of　the　ATNs　as　negative　electrode　for　lithium-ion　batteries　is　investigated　by　the　galvanostatic　chronopotentiometry　and　cyclic　voltammetry.　A　high　initial　discharge　capacity　(311　mA　h　g(-1))　and　initial　Coulombic　efficiency　(81.7%)　were　obtained　for　ATNs,　and　capacities　of　252,　202,　186,　158,　136,　and　119　mA　h　g(-1)　were　obtained　at　0.2,　1,　5,　10,　20,　and　30　C,　respectively.　Particularly,　the　ATNs　can　still　maintains　a　capacity　of　108　mA　h　g(-1)　after　4000　cycles　at　30　C　(only　a　capacity　loss　of　10%),　which　indicated　a　superior　rate　capabilities　and　cyclability.　The　CVs　analysis　revealed　that　the　ANTs　have　both　diffusive　lithium　storage　in　the　bulk　and　pseudocapacitive　lithium　storage　at　the　surface　(also　called　interfacial　lithium　storage),　and　the　pseudocapacitive　lithium　storage　dominates　the　total　capacity　when　the　scan　rates　are　above　1　mV　s(-1).　The　fast　and　stable　lithium　storage　of　ATNs　might　be　attributed　to　the　high　pseudocapacitive　lithium　storage　contribution　in　the　material,　and　it　was　suggested　the　pseudocapacitive　lithium　storage　could　occurred　at　grain-grain　interfaces　as　well　as　nanosheet　surfaces.