Chitosan-stavudine　(d4T)　conjugate　with　a　succinic　spacer　was　synthesized　via　carbodiimide　coupling　reaction　and　structurally　characterized.　In　order　to　nanosize　it　for　improving　its　therapeutic　properties,　the　chitosan-5＇-O-succinyl-d4T　conjugate　was　crosslinked　with　sodium　tripolyphosphate　(TPP)　to　obtain　the　chitosan-d4T　conjugate　nano-prodrug.　The　morphologies　of　chitosan-d4T　conjugate　nanoparticles　were　observed　by　transmission　electron　microscopy　(TEM),　and　their　zeta　potential,　particle　size,　and　polydispersity　(size　distribution)　were　measured　by　the　dynamic　light　scattering　(DLS)　techniques.　In　vitro　drug　release　studies　at　pH　1.1　and　pH　7.4　indicate　that　the　crosslinked　chitosan-d4T　conjugate　nano-prodrug　can　prevent　the　coupled　d4T　from　leaking　out　before　entering　the　target　viral　reservoirs　and　provide　a　mild　sustained　release　without　the　burst　release.　The　results　reveal　that　constructing　conjugated　chitosan　nano-prodrugs　may　be　a　promising　approach　for　improving　the　therapy　efficacy　of　drugs　in　antiviral　treatment.