This　work　reports　that　membranes　were　cast　from　solution　consisting　of　dimethylformamide,　polyethylene　glycol,　and　polyvinyl　chloride-co-vinyl　acetate　(VC-co-VAc-OH)　to　prepare　flat-sheet　membranes　on　the　nonwoven　fabrics　via　phase　inversion　method.　The　influence　of　various　factors　(solid　content,　the　content　of　additive,　environmental　temperature,　relative　humidity,　evaporation　time　of　solvent,　coagulation　temperature,　and　coagulation　time)　on　membrane　performance　(the　pure　water　flux　and　the　retention　of　bovine　serum　albumin　(BSA))　were　studied　in　the　film-forming　process　of　the　VC-co-VAc-OH　membrane.　The　results　showed　that　the　membrane　with　higher　pure　water　flux　(289.6　mL　cm(-2)　h(-1))　and　good　retention　of　BSA　(86.7%)　can　be　prepared　under　optimized　preparation　conditions.　In　addition,　the　carbon　dioxide　gas,　which　was　produced　by　a　chemical　reaction　between　glacial　acetic　acid　in　the　casting　solution　and　sodium　carbonate　in　coagulation　medium,　can　improve　the　antifouling　ability　of　the　membrane.　The　membrane　prepared　by　chemical　reaction　under　optimum　conditions　(the　molar　ratio　between　Na2CO3　and　glacial　acetic　acid　is　0.5:1)　possess　higher　pure　water　flux　(457.7　mL　cm(-2)　h(-1))　and　good　retention　of　BSA　(90.7%).