Adenovirus-mediated bone morphogenetic protein-2 gene transfection of bone marrow mesenchymal stem cells combined with nano-hydroxyapatite to construct bone graft material in vitro - 文章详情页

作者：

Li, W. C. (Li, W. C..) | Wang, D. P. (Wang, D. P..) | Li, L. J. (Li, L. J..) | Zhu, W. M. (Zhu, W. M..) | Zeng, Y. J. (Zeng, Y. J..)

收录：

EI Scopus SCIE

摘要：

To　study　the　adhesion,　proliferation　and　expression　of　bone　marrow　mesenchymal　stem　cells　(BMSCs)　on　nano-hydroxyapatite　(Nano-HA)　bone　graft　material　after　transfection　of　adenovirus-mediated　human　bone　morphogenetic　protein-2　expression　vector　(Ad-BMP-2).　BMSCs　were　transfected　using　Ad-BMP-2.　Immunohistochemistry　and　Western　blot　were　used　to　detect　BMP-2　expression　in　transfected　cells.　After　transfection,　BMP-2　protein　was　highly　expressed　in　BMSCs;　MTT　test　assay　showed　that　the　Nano-HA　bone　graft　material　could　not　inhibit　in　vitro　proliferation　of　BMSCs.　Ad-BMP-2-transfected　BMSCs　are　well　biocompatible　with　Nano-HA　bone　graft　material,　the　transfected　cells　in　material　can　secrete　BMP-2　stably　for　a　long　time.

关键词：

nano-hydroxyapatite adenoviral vector marrow-derived mesenchymal stem cell bone morphogenetic protein tissue-engineered bone

作者机构：

[ 1 ] [Li, W. C.]Second Peoples Hosp Shenzhen, Dept Trauma Orthoped, Shenzhen 518035, Guangdong, Peoples R China
[ 2 ] [Wang, D. P.]Second Peoples Hosp Shenzhen, Dept Trauma Orthoped, Shenzhen 518035, Guangdong, Peoples R China
[ 3 ] [Li, L. J.]Second Peoples Hosp Shenzhen, Dept Trauma Orthoped, Shenzhen 518035, Guangdong, Peoples R China
[ 4 ] [Zhu, W. M.]Second Peoples Hosp Shenzhen, Dept Trauma Orthoped, Shenzhen 518035, Guangdong, Peoples R China
[ 5 ] [Zeng, Y. J.]Beijing Univ Technol, Biomech & Med Informat Inst, Beijing, Peoples R China

通讯作者信息：

[Wang, D. P.]Second Peoples Hosp Shenzhen, Dept Trauma Orthoped, Shenzhen 518035, Guangdong, Peoples R China

电子邮件地址：

szhzwm@126.com

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