In　this　paper,　novel　cyclosporine　A　(CsA)-loaded　amphiphilic　poly(L-aspartic　acid-co-L-lactic　acid)　(PAL)-1,2-dipalmitoyl-sn-glycero-3-phosphoethanolamine　(DPPE)　copolymer　nanoparticles　(NPs)　were　successfully　fabricated　using　an　emulsion/solvent　evaporation　technique.　The　CsA-loaded　NPs　were　characterized　by　dynamic　light　scattering　(DLS)　and　transmission　electron　microscopy　(TEM).　The　CsA-loaded　NPs　size,　size　distribution,　encapsulation　efficiency　(EE)　and　drug-loading　content　(LC)　were　influenced　by　polyvinyl　alcohol　(PVA)　concentration　and　the　weight　ratio　of　the　copolymer　to　CsA.　In　vitro　release　behavior　of　CsA-loaded　NPs　showed　a　sustained　release.　With　the　increasing　of　copolymer/CsA　weight　ratio,　the　release　of　CsA　from　NPs　is　rapid.　The　poly(L-aspartic　acid)　derivatives　NPs　have　a　promising　potential　in　hydrophobic　drug　delivery　system.