The　objective　of　present　paper　is　to　study　the　immunogenicity　of　combinations　of　multiple　vector　vaccines　expressing　HIV-1　structural　genes　from　different　subtypes.　Mice　were　vaccinated　with　DNA　(B＇/C)　and　rMVA　(B＇/C)　vaccines　expressing　B＇/C　recombinant　subtype　gag-pol　and　env　genes,　DNA　(B＇)　and　rAd5　(B＇)　vaccines　expressing　subtype　B＇　gag　gene　with　different　combination　schemes.　HIV-1　Gag-specific　cellular　immune　responses　and　P24-specific　IgG　levels　were　analyzed　by　IFN-gamma　enzyme-linked　immunospot　assay　(ELISPOT)　and　enzyme-linked　immunosorbent　assay　(ELISA)　respectively.　ELISPOT　results　indicated　that　the　Gag-specific　cellular　immune　responses　induced　by　combination　of　three　vaccines　were　much　higher　than　that　induced　by　combination　of　two　vaccines.　Among　the　groups　of　mice　immunized　with　two　vaccines,　the　groups　with　rAd5　booster　elicited　higher　cellular　immune　responses　compared　with　the　groups　with　rMVA　booster.　All　the　test　groups　of　three　vaccines　in　combination　could　induce　similar　level　of　cellular　immune　responses,　which　did　not　correlate　with　the　immunization　order.　ELISA　results　showed　that　p24-specific　IgG　induced　by　combination　of　three　vaccines　were　much　higher　than　that　induced　by　combination　of　two　vaccines.　It　indicates　that　the　combination　scheme　of　multiple　vector　vaccines　maybe　a　promising　AIDS　vaccine　strategy.