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In order to optimize the nitrogen removal efficiency of the anoxic/oxic (A/O) process in a state of limited filamentous bulking, both an A/O reactor and lab-scale sequencing batch reactors were used to perform experiments on anoxic denitrification. Methanol, ethanol, sodium acetate, and sodium propionate were used as external carbon sources. The results showed that the highest specific denitrification rate (7.06 mgN/(gSS.h)) was achieved when sodium acetate was added to SBRs. When external carbon sources were added to the anoxic zone during the A/O process, the average total nitrogen (TN) removal efficiency rose to 56.5% and the mean concentration of NO2--N in effluent from the anoxic zone was 6.73 mg/L. When the hydraulic retention time (HRT) was extended to 11 h 10 min (the actual retention time of anoxic zone was 45 min), the average TN removal efficiency reached 70.4% and the mean concentration of NO2--N in effluent fell to 2.61 mg/L. The results of this study indicated that massive nitrite accumulation was caused by some carbon sources and the lack of actual HRT in the anoxic zone.
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