Infrared　spectroscopy　(IR)　is　employed　as　an　important　tool　in　the　investigation　of　biological　macromolecules　because　of　its　high　sensitivity　and　nondestructivity　to　samples.　When　proteins,　lipids　or　nucleic　acids　are　damaged,　the　position,　shape　and　intensity　of　their　IR　characteristic　absorption　peaks　will　be　significantly　changed.　This　provides　evidences　for　the　determination　of　the　damages　of　biomolecules,　which　further　shed　light　on　the　clarification　of　the　occurrence,　development　and　early　prevention　of　some　diseases.　In　the　present　paper,　the　applications　of　IR　to　the　detection　of　biomolecular　damages　are　reviewed.　Various　IR　techniques　are　introduced,　including　Fourier　transform　infrared　spectroscopy　(FTIR),　attenuated　total　reflection　Fourier　transform　infrared　spectroscopy　(ATR-FTIR),　Fourier　transform　infrared　spectroscopy　microscope　and　so　on.　A　general　review　was　performed　for　the　application　of　IR　technique　to　the　qualitative　and　quantitative　analysis　of　secondary　structure　of　protein,　fluidity　of　membrane　lipid,　interaction　of　drugs　with　DNA　and　so　on.　The　existing　problems　of　IR　were　pointed　out　and　the　prospect　of　IR　technique　in　the　field　of　biomedicine　was　discussed.　It　is　expected　that,　in　the　future　the　researches　involving　IR　techniques　will　be　focused　on　the　application　in　early　diagnosis　of　diseases,　instrumental　combinations　and　quantitative　techniques.