This　paper　reports　an　investigation　of　the　characteristics　of　human　dentine　ablated　by　a　femtosecond　laser　of　800　nm　wavelength　for　potential　pain　free　dental　restoration　applications.　The　work　has　shown　that　precise　femtosecond　laser　ablation　with　clear　boundaries　and　without　cracks　and　debris　can　be　obtained.　The　transmittance　of　the　dentine　tissue　was　similar　to　2.7%,　and　the　absorption　coefficient　was　similar　to　3.19　cm(-1)　at　800　nm.　The　laser　processed　dentine　morphology　was　investigated　over　a　wide　range　of　laser　fluences　and　pulse　numbers.　The　fluence　threshold　of　dentine　was　determined　as　0.45　J　cm(-2)　according　to　the　experimental　dependence　of　the　ablated　depth　versus　laser　fluence.　The　thermal　energy　conduction　effects　of　dentine　tubules　were　analysed.　The　SEM-EDX　and　Raman　mapping　analysis　of　phosphate　indicate　that　the　chemical　components　of　the　dentine　remained　intact　before　and　after　ablation.